Description
This assay is a one-step lateral flow chromatographic immunoassay. The test strip consists of: 1) a burgundy colored
conjugate pad containing mouse anti-hHb antibodies conjugated with colloidal gold and 2) a nitrocellulose membrane strip containing a Test line (T-line) and a Control line (C-line). The Tline is coated with anti-hHb antibodies, and the C-line is coated with goat anti-mouse IgG antibodies.
When an adequate volume of test specimen is dispensed into the sample well of the device, the test specimen migrates by capillary action across the test strip. If the concentration of hHb in the specimen is at or above 50 ng/ml, the T-line appears as a visible burgundy line. If the concentration of hHb in the specimen is below
the detectable level, no T-line develops.
The C-line is coated with goat anti-mouse antibody, which binds to the conjugated monoclonal antibody, regardless of the presence of hHb in the sample.
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